Characterization of Lactobacillus plantarum PH04, a potential probiotic bacterium with cholesterol-lowering effects

Int J Food Microbiol. 2007 Feb 15;113(3):358-61. doi: 10.1016/j.ijfoodmicro.2006.08.015. Epub 2006 Nov 29.


The bacterium Lactobacillus plantarum PH04 was isolated from infant feces and tested positive for bile/acid tolerance and bile salt hydrolase activity. It was evaluated as a potential probiotic with cholesterol-lowering effect. Bile salt hydrolase activity was nine times greater in stationary phase than in exponential phase cells and increased when the cells were exposed to conjugated bile salts. L. plantarum PH04 was resistant to seven of nine antibiotics tested and did not produce beta-glucuronidase. L. plantarum PH04 was fed to hypercholesterolemic mice at numbers of 10(7) CFU per mouse per day for 14 days. Compared with a control group, the serum cholesterol and triglycerides were respectively 7 and 10% lower in the group fed L. plantarum PH04, and fecal lactic acid bacteria increased while no any significant differences (P<0.05) in body weight, visceral weigh index or bacteria translocation between two groups were observed. The results indicated that L. plantarum PH04 might be effective as a probiotic with cholesterol-lowering activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / metabolism*
  • Animals
  • Anticholesteremic Agents / pharmacology*
  • Anticholesteremic Agents / therapeutic use
  • Biological Assay
  • Cholesterol / blood
  • Cholesterol / metabolism*
  • Colony Count, Microbial
  • Feces / microbiology
  • Lactobacillus plantarum / enzymology
  • Lactobacillus plantarum / growth & development
  • Lactobacillus plantarum / isolation & purification
  • Lactobacillus plantarum / physiology*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Probiotics / pharmacology*
  • Probiotics / therapeutic use
  • Random Allocation
  • Triglycerides / blood
  • Triglycerides / metabolism


  • Anticholesteremic Agents
  • Triglycerides
  • Cholesterol
  • Amidohydrolases