Setup and characterization of a human head and neck squamous cell carcinoma xenograft model in nude rats

Otolaryngol Head Neck Surg. 2006 Dec;135(6):853-7. doi: 10.1016/j.otohns.2006.06.1257.

Abstract

Objective: To develop and characterize a new head and neck cancer animal model.

Study design: A human head and neck squamous cell carcinoma (HNSCC) xenograft model in nude rats was established via subcutaneous inoculation of a human-origin HNSCC cell line, SCC-4. The tumor was evaluated for growth characteristics, pathologic features by hematoxylin-eosin (HE) staining, and immunohistochemistry of epidermal growth factor receptor (EGFR). 2-[18F] fluoro-2-deoxy-D-glucose (18F-FDG) positron emission tomography (PET) imaging characteristics were studied too.

Results: A new HNSCC animal model was successfully established. Tumor sizes reached about 1 cm3 on day 15 after tumor cell inoculation. HE staining pathology has confirmed that this tumor is a typical SCC. EGFR immunohistochemistry demonstrated this tumor model to be strongly EGFR positive. 18F-FDG PET study has shown that 18F-FDG accumulated in tumors.

Conclusions: This study has demonstrated that this tumor model is an appropriate HNSCC tumor model for animal studies on HNSCC.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Carcinoma, Squamous Cell / diagnostic imaging
  • Carcinoma, Squamous Cell / pathology*
  • Cell Line, Tumor
  • Disease Models, Animal*
  • Fluorodeoxyglucose F18
  • Head and Neck Neoplasms / diagnostic imaging
  • Head and Neck Neoplasms / pathology*
  • Humans
  • Positron-Emission Tomography
  • Radiography
  • Radiopharmaceuticals
  • Rats
  • Rats, Nude*
  • Transplantation, Heterologous

Substances

  • Radiopharmaceuticals
  • Fluorodeoxyglucose F18