The anaphase-promoting complex is required in both dividing and quiescent cells during zebrafish development

Dev Biol. 2007 Mar 1;303(1):144-56. doi: 10.1016/j.ydbio.2006.10.043. Epub 2006 Nov 3.


The anaphase-promoting complex/cyclosome (APC/C) regulates multiple stages of the cell cycle, most prominently mitosis. We describe zebrafish with mutations in two APC/C subunits, Cdc16 and Cdc26, whose phenotypes reveal a multifaceted set of defects resulting from the gradual depletion of the APC/C. First, loss of the APC/C in dividing cells results in mitotic arrest, followed by apoptosis. This defect becomes detectable in different organs at different larval ages, because the subunits of the APC/C are maternally deposited, are unusually stable, and are depleted at uneven rates in different tissues. Second, loss of the APC/C in quiescent or differentiated cells results in improper re-entry into the cell cycle, again in an apparently tissue-specific manner. This study is the first demonstration of both functions of the APC/C in a vertebrate organism and also provides an illustration of the surprisingly complex effects that essential, maternally supplied factors can have on the growing animal over a period of 10 days or longer.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Animals
  • Base Sequence
  • Cell Cycle / physiology*
  • Cell Cycle Proteins / genetics
  • Chromosome Mapping
  • Cyclin B
  • DNA Primers
  • Immunohistochemistry
  • Mitosis / physiology*
  • Models, Biological*
  • Molecular Sequence Data
  • Retina / embryology
  • Sequence Analysis, DNA
  • Ubiquitin-Protein Ligase Complexes / genetics*
  • Ubiquitin-Protein Ligase Complexes / metabolism*
  • Zebrafish / embryology*


  • Cell Cycle Proteins
  • Cyclin B
  • DNA Primers
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome