Tolerance of transgenic canola plants (Brassica napus) amended with plant growth-promoting bacteria to flooding stress at a metal-contaminated field site

Environ Pollut. 2007 Jun;147(3):540-5. doi: 10.1016/j.envpol.2006.10.014. Epub 2006 Dec 4.

Abstract

The growth of transgenic canola (Brassica napus) expressing a gene for the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase was compared to non-transformed canola exposed to flooding and elevated soil Ni concentration, in situ. In addition, the ability of the plant growth-promoting bacterium Pseudomonas putida UW4, which also expresses ACC deaminase, to facilitate the growth of non-transformed and transgenic canola under the above mentioned conditions was examined. Transgenic canola and/or canola treated with P. putida UW4 had greater shoot biomass compared to non-transformed canola under low flood-stress conditions. Under high flood-stress conditions, shoot biomass was reduced and Ni accumulation was increased in all instances relative to low flood-stress conditions. This is the first field study to document the increase in plant tolerance utilizing transgenic plants and plant growth-promoting bacteria exposed to multiple stressors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Brassica napus / genetics
  • Brassica napus / growth & development*
  • Brassica napus / microbiology
  • Carbon-Carbon Lyases / genetics
  • Disasters
  • Environmental Exposure / adverse effects
  • Gene Expression
  • Germination / genetics
  • Germination / physiology
  • Nickel / toxicity*
  • Plant Roots / chemistry
  • Plant Shoots / chemistry
  • Plant Shoots / growth & development
  • Plants, Genetically Modified / growth & development*
  • Plants, Genetically Modified / microbiology
  • Pseudomonas putida / genetics
  • Pseudomonas putida / physiology*
  • Seeds / genetics
  • Seeds / growth & development
  • Seeds / microbiology
  • Soil / analysis
  • Soil Pollutants / toxicity*

Substances

  • Soil
  • Soil Pollutants
  • Nickel
  • 1-aminocyclopropane-1-carboxylate deaminase
  • Carbon-Carbon Lyases