Tomographic molecular imaging and 3D quantification within adult mouse organs

Nat Methods. 2007 Jan;4(1):31-3. doi: 10.1038/nmeth985. Epub 2006 Dec 3.


A convenient technology to quantify three-dimensional (3D) morphological features would have widespread applications in biomedical research. Based on combined improvements in sample preparation, tomographic imaging and computational processing, we present a procedure for high-resolution 3D quantification of structures within intact adult mouse organs. Using the nonobese diabetic (NOD) mouse model, we demonstrate a correlation between total islet beta-cell volume and the onset of type-1 diabetes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Diabetes Mellitus, Type 1 / diagnosis*
  • Diabetes Mellitus, Type 1 / physiopathology
  • Disease Models, Animal
  • Disease Progression
  • Image Processing, Computer-Assisted / methods*
  • Imaging, Three-Dimensional / methods*
  • Islets of Langerhans / pathology*
  • Mice
  • Pancreas / pathology*
  • Sensitivity and Specificity
  • Tomography / methods*