Mutational Analysis of Chk1, Chk2, Apaf1 and Rb1 in Human Malignant Melanoma Cell Lines

Oncol Rep. 2007 Jan;17(1):135-40.

Abstract

Four tumour suppressor genes (Chk1, Chk2, Apaf1 and Rb1) in nine human malignant melanoma cell lines were screened for a loss of gene expression, point mutations and small deletions/insertions by cDNA-based DGGE/SCCP analysis. In two cell lines alterations of the investigated genes could be demonstrated. This result confirms our assumption of the participation of dysfunctional p53 inducer/effector genes in human melanoma aetiology. Furthermore, it points towards the probable principal role of diverse alternative p53-pathway disruption mechanisms in this highly therapy-resistant malignancy without affecting p53 itself. To our knowledge, this is the first CHK1/CHK2 mutation screening in human melanoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptotic Protease-Activating Factor 1 / genetics*
  • Base Sequence
  • Cell Line, Tumor
  • Checkpoint Kinase 1
  • Checkpoint Kinase 2
  • Codon
  • DNA Mutational Analysis
  • Genes, Tumor Suppressor*
  • Humans
  • Melanoma / genetics*
  • Molecular Sequence Data
  • Mutation*
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases / genetics*
  • Retinoblastoma Protein / genetics*

Substances

  • APAF1 protein, human
  • Apoptotic Protease-Activating Factor 1
  • Codon
  • Retinoblastoma Protein
  • Protein Kinases
  • Checkpoint Kinase 2
  • CHEK1 protein, human
  • CHEK2 protein, human
  • Checkpoint Kinase 1
  • Protein-Serine-Threonine Kinases