Evaluation of renal cell carcinoma vaccines targeting carbonic anhydrase IX using heat shock protein 110

Cancer Immunol Immunother. 2007 Jul;56(7):1097-105. doi: 10.1007/s00262-006-0258-z. Epub 2006 Dec 5.


Carbonic anhydrase IX (CA9) is a renal cell carcinoma (RCC)-specific tumor protein that is targeted using heat shock protein 110 (hsp110). The chaperoning ability of hsp110 can be utilized to form a complex with CA9 (hsp110 + CA9) in vitro, which can be administered as a highly concentrated tumor vaccine. In a tumor prevention model, hsp110 + CA9 prevented the growth of RENCA tumors in BALB/c mice, and produced IFN-gamma response measured using ELISPOT and an antibody response measured using ELISA. To test a second vaccine strategy, hsp110 complexed to a previously described CA9 peptide prevented tumor growth and produced a very weak IFN-gamma response, but no antibody response. A plasmid vector containing grp170, a member of the hsp110 family, linked to CA9 did not produce an antitumor response and produced no IFN-gamma response or antibodies. In a model of metastatic RCC, RENCA cells were injected intradermally prior to vaccination. Hsp110 + CA9 decreased tumor growth compared to control vaccinations. These studies suggest that recombinant hsp110 complexed to CA9 should be evaluated for treatment of RCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Neoplasm / immunology
  • Antigens, Neoplasm / therapeutic use
  • Cancer Vaccines / immunology
  • Cancer Vaccines / therapeutic use*
  • Carbonic Anhydrases / immunology*
  • Carbonic Anhydrases / therapeutic use
  • Carcinoma, Renal Cell / immunology
  • Carcinoma, Renal Cell / therapy*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genetic Vectors
  • HSP110 Heat-Shock Proteins / immunology*
  • HSP110 Heat-Shock Proteins / therapeutic use
  • Humans
  • Immunotherapy / methods*
  • Kidney Neoplasms / immunology
  • Kidney Neoplasms / therapy*
  • Mice
  • Mice, Inbred BALB C
  • Plasmids / genetics
  • Recombinant Proteins / immunology


  • Antigens, Neoplasm
  • Cancer Vaccines
  • HSP110 Heat-Shock Proteins
  • Recombinant Proteins
  • Carbonic Anhydrases