Purification and characterization of a corrinoid-compound in an edible cyanobacterium Aphanizomenon flos-aquae as a nutritional supplementary food

J Agric Food Chem. 2006 Dec 13;54(25):9604-7. doi: 10.1021/jf062300r.


The vitamin B12 concentration of the dried cells of Aphanizomenon flos-aquae was determined by both microbiological method with Lactobacillus delbrueckeii ATCC7830 and chemiluminescence method with intrinsic factor. The Aphanizomenon cells contained 616.3 +/- 30.3 micro g (n = 4) of vitamin B12 per 100 g of the dried cells by the microbiological method. The values determined with the chemiluminescence method, however, were only about 5.3% of the values determined by the microbiological method. A corrinoid-compound was purified from the dried cells and characterized. The purified corrinoid-compound was identified as pseudovitamin B12 (an inactive corrinoid-compound for humans) by silica gel 60 TLC, C18 reversed-phase HPLC, ultraviolet-visible spectroscopy, and 1H NMR spectroscopy. The results suggest that the Aphanizomenon cells are not suitable for use as a vitamin B12 source, especially in vegans.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aphanizomenon / chemistry*
  • Chromatography, High Pressure Liquid
  • Corrinoids / isolation & purification*
  • Dietary Supplements*
  • Magnetic Resonance Spectroscopy
  • Vitamin B 12 / analysis


  • Corrinoids
  • Vitamin B 12