Sequence polymorphism of the mitochondrial DNA hypervariable regions I and II in 205 Singapore Malays

Leg Med (Tokyo). 2007 Jan;9(1):33-7. doi: 10.1016/j.legalmed.2006.08.007. Epub 2006 Dec 5.

Abstract

Mitochondrial DNA sequences of the hypervariable regions HV1 and HV2 were analyzed in 205 unrelated ethnic Malays residing in Singapore as an initial effort to generate a database for forensic identification purposes. Sequence polymorphism was detected using PCR and direct sequencing analysis. A total of 152 haplotypes was found containing 152 polymorphisms. Out of the 152 haplotypes, 115 were observed only once and 37 types were seen in multiple individuals. The most common haplotype (16223T, 16295T, 16362C, 73G, 146C, 199C, 263G, and 315.1C) was shared by 7 (3.41%) individuals, two haplotypes were shared by 4 individuals, seven haplotypes were shared by 3 individuals, and 27 haplotypes by 2 individuals. Haplotype diversity and random match probability were estimated to be 0.9961% and 0.87%, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Complementarity Determining Regions / genetics*
  • DNA Fingerprinting
  • DNA, Mitochondrial / genetics*
  • Ethnicity / ethnology*
  • Genetics, Population*
  • Haplotypes
  • Humans
  • Malaysia / ethnology
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA
  • Singapore

Substances

  • Complementarity Determining Regions
  • DNA, Mitochondrial