An empirical approach for thermal stability (Tm) prediction of PNA/DNA duplexes

Toshiyuki Takiya; Yasuhiro Seto; Hideyo Yasuda; Tohru Suzuki; Keiichi Kawai

doi:10.1093/nass/48.1.131

An empirical approach for thermal stability (Tm) prediction of PNA/DNA duplexes

Nucleic Acids Symp Ser (Oxf). 2004:(48):131-2. doi: 10.1093/nass/48.1.131.

Authors

Toshiyuki Takiya¹, Yasuhiro Seto, Hideyo Yasuda, Tohru Suzuki, Keiichi Kawai

Affiliation

¹ Nippon Flour Mills Co., Ltd., 5-1-3 Midorigaoka, Atsugi 243-0041, Japan.

PMID: 17150513
DOI: 10.1093/nass/48.1.131

Abstract

To predict the Tm value of PNA/DNA duplex, we performed to determine the melting temperature of several PNA oligomers. For Tm calculation model building, we searched the correlativity of measured Tm value and each base contents of PNA oligomer. Here, We propose a novel formula for thermal stability (Tm) prediction of PNA/DNA duplexes.

MeSH terms

Base Composition
DNA / chemistry*
Empirical Research*
Nucleic Acid Denaturation
Nucleic Acid Heteroduplexes / chemistry*
Peptide Nucleic Acids / chemistry*
Thermodynamics
Transition Temperature

Substances

Nucleic Acid Heteroduplexes
Peptide Nucleic Acids
DNA