Translesion Synthesis by Human DNA Polymerase Eta Across Oxidative Products of Guanine

Nucleic Acids Symp Ser (Oxf). 2004;(48):171-2. doi: 10.1093/nass/48.1.171.

Abstract

Guanine is the most oxidizable base among natural bases. 8-Oxoguanine (8-oxoG) is the typical oxidative product, but the amount of 8-oxoG does not directly reflect the strength of oxidative stress. Imidazolone, oxazolone and guanidinohydantoin are oxidative products of guanine and 8-oxoG. Here, we investigated enzymatic reactions with human DNA polymerase eta on these lesions.

MeSH terms

  • Base Sequence
  • DNA Damage*
  • DNA-Directed DNA Polymerase / metabolism*
  • Guanine / chemistry
  • Guanine / metabolism*
  • Humans
  • Molecular Sequence Data
  • Nucleotides / metabolism
  • Oxidation-Reduction
  • Substrate Specificity

Substances

  • Nucleotides
  • Guanine
  • DNA-Directed DNA Polymerase
  • Rad30 protein