SNPs typing by base-discriminating fluorescence DNA probe

Nucleic Acids Symp Ser (Oxf). 2005:(49):201-2. doi: 10.1093/nass/49.1.201.

Abstract

For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present SNP typing method with BDF probes is a very powerful homogeneous assay that does not require a special device or time-consuming steps.

Publication types

  • Evaluation Study

MeSH terms

  • DNA Probes / chemistry*
  • Deoxycytidine / analogs & derivatives*
  • Deoxycytidine / chemistry
  • Deoxyuridine / analogs & derivatives*
  • Deoxyuridine / chemistry
  • Fluorescent Dyes / chemistry*
  • Genes, BRCA1
  • Humans
  • Nucleic Acid Hybridization / methods*
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide*
  • Pyrimidine Nucleosides / chemistry

Substances

  • 4-N-(N,N-dimethylaminomethylidenyl)-5-(3-(1-pyrenecarboxamido)propynyl)-5'-O-(4,4-dimethoxytrityl)-2'-deoxycytidine
  • 5-(3-(1-pyrenecarboxamido)propynyl)-2'-deoxyuridine
  • DNA Probes
  • Fluorescent Dyes
  • Pyrimidine Nucleosides
  • Deoxycytidine
  • Deoxyuridine