Abstract
For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present SNP typing method with BDF probes is a very powerful homogeneous assay that does not require a special device or time-consuming steps.
MeSH terms
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DNA Probes / chemistry*
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Deoxycytidine / analogs & derivatives*
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Deoxycytidine / chemistry
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Deoxyuridine / analogs & derivatives*
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Deoxyuridine / chemistry
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Fluorescent Dyes / chemistry*
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Genes, BRCA1
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Humans
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Nucleic Acid Hybridization / methods*
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Polymerase Chain Reaction
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Polymorphism, Single Nucleotide*
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Pyrimidine Nucleosides / chemistry
Substances
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4-N-(N,N-dimethylaminomethylidenyl)-5-(3-(1-pyrenecarboxamido)propynyl)-5'-O-(4,4-dimethoxytrityl)-2'-deoxycytidine
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5-(3-(1-pyrenecarboxamido)propynyl)-2'-deoxyuridine
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DNA Probes
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Fluorescent Dyes
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Pyrimidine Nucleosides
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Deoxycytidine
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Deoxyuridine