Abstract
Acute pancreatic oedema with hyperamylasaemia was induced in mice by subcutaneous administration of cholecystokinin octapeptide (CCK8). Comparison with effect of caerulein showed that cholecystokinin is less potent in vivo. To investigate the observed difference in response, threonine3 CCK8 and methionine5 caerulein were synthesised and evaluated. Comparison of these peptides suggests that difference in bioactivity is related to possession of extra N-terminal residues rather than substitution of threonine for methionine.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amylases / blood
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Animals
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Ceruletide / administration & dosage
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Ceruletide / chemistry
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Ceruletide / metabolism
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Ceruletide / pharmacology*
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Dose-Response Relationship, Drug
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Injections, Subcutaneous
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Male
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Methionine / metabolism
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Mice
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Mice, Inbred CBA
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Molecular Sequence Data
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Molecular Structure
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Pancreatitis / chemically induced
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Pancreatitis / metabolism*
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Sincalide / administration & dosage
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Sincalide / chemistry
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Sincalide / metabolism
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Sincalide / pharmacology*
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Threonine / metabolism
Substances
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Threonine
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Ceruletide
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Methionine
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Amylases
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Sincalide