Nucleotide insertion opposite a cyclobutane pyrimidine dimer analogue caused from photoligation by a replicative DNA polymerase

Nucleic Acids Symp Ser (Oxf). 2006;(50):125-6. doi: 10.1093/nass/nrl062.

Abstract

We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine ((CV)U)-containing ODN at the 5'-terminal. This method forms d(T-(CV)U) as a cyclobutane pyrimidine dimer (CPD) analogue between 3'-terminal thymidine and 5'-terminal (CV)U of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-(CV)U). And then, we disclosed that two adenines were incorporated opposite the d(T-(CV)U).

MeSH terms

  • Adenine / metabolism
  • DNA / biosynthesis*
  • DNA / chemistry
  • Deoxyuridine / analogs & derivatives*
  • Deoxyuridine / chemistry
  • Electrophoresis, Microchip
  • Oligodeoxyribonucleotides / chemistry
  • Oligodeoxyribonucleotides / radiation effects
  • Photochemistry
  • Polymerase Chain Reaction
  • Pyrimidine Dimers / chemistry*
  • Taq Polymerase / metabolism*
  • Templates, Genetic
  • Thymidine / analogs & derivatives*
  • Thymidine / chemistry

Substances

  • Oligodeoxyribonucleotides
  • Pyrimidine Dimers
  • DNA
  • Taq Polymerase
  • Adenine
  • Thymidine
  • Deoxyuridine