Electrochemical detection of DNase I activity

Katsuya Fujita; Masanori Kanazawa; Kosuke Mukumoto; Takahiko Nojima; Shinobu Sato; Hiroki Kondo; Michinori Waki; Shigeori Takenaka

doi:10.1093/nass/nrl153

Electrochemical detection of DNase I activity

Nucleic Acids Symp Ser (Oxf). 2006:(50):307-8. doi: 10.1093/nass/nrl153.

Authors

Katsuya Fujita¹, Masanori Kanazawa, Kosuke Mukumoto, Takahiko Nojima, Shinobu Sato, Hiroki Kondo, Michinori Waki, Shigeori Takenaka

Affiliation

¹ Department of Materials Science, Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensui-cyo, Tobata-ku, Kitakyushu-shi, Fukuoka 804-8550, Japan.

PMID: 17150940
DOI: 10.1093/nass/nrl153

Abstract

DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbodiimides / chemistry*
Deoxyribonuclease I / analysis*
Electrochemistry / instrumentation
Electrochemistry / methods*
Electrodes
Ferrous Compounds / chemistry*
Oligonucleotides / chemistry
Oxidation-Reduction
Thionucleotides / chemistry

Substances

Carbodiimides
Ferrous Compounds
Oligonucleotides
Thionucleotides
ferrocenyl carbodiimide
Deoxyribonuclease I