Controlling the expression ratio of two proteins by inserting a terminator between the two genes

Nucleic Acids Symp Ser (Oxf). 2006:(50):329-30. doi: 10.1093/nass/nrl164.


An approach was developed in controlling the expression ratio of two genes encoded in DNA by inserting a terminator sequence between the two genes. Escherichia coli ribosomal RNA operon T1 terminator, or E. coli tryptophane attenuater was introduced between two green fluorescent protein (GFP) mutants: ultraviolet-optimized mutant (GFPuv: F99S, M153T, V163A) and blue-shifted mutant (BFP: F64L, S65T, T145F). The ratio of the fluorescence intensity of BFP to GFPuv after transcription and translation reflected the termination efficiency of the transcriptional termination activity of the inserted terminator.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / genetics
  • Gene Expression Regulation*
  • Green Fluorescent Proteins / biosynthesis
  • Green Fluorescent Proteins / genetics
  • Luminescent Agents / metabolism
  • Protein Biosynthesis*
  • Terminator Regions, Genetic*
  • Transcription, Genetic


  • Luminescent Agents
  • Green Fluorescent Proteins