Abstract
Two Pseudomonas aeruginosa strains resistant to beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems and susceptible only to polymyxin B (MIC <or= 2 microg/ml) were identified as part of the Meropenem Yearly Susceptibility Test Information Collection program. Metallo-beta-lactamase screening tests were positive, PCR yielded products with blaVIM primers, and sequence analysis revealed blaVIM-7 and blaVIM-2. The isolates had distinct ribotype and pulsed-field gel electorphoresis patterns and appeared independently, remote in time and location, at the same cancer center.
MeSH terms
-
Academic Medical Centers
-
Anti-Bacterial Agents / pharmacology
-
Bacterial Proteins / biosynthesis*
-
Bacterial Proteins / genetics
-
Drug Resistance, Multiple, Bacterial*
-
Humans
-
Microbial Sensitivity Tests / methods
-
Pseudomonas Infections / epidemiology
-
Pseudomonas Infections / microbiology
-
Pseudomonas aeruginosa / drug effects*
-
Pseudomonas aeruginosa / enzymology*
-
Pseudomonas aeruginosa / genetics
-
Pseudomonas aeruginosa / isolation & purification
-
Texas
-
beta-Lactam Resistance
-
beta-Lactamases / biosynthesis*
-
beta-Lactamases / genetics
Substances
-
Anti-Bacterial Agents
-
Bacterial Proteins
-
beta-lactamase bla(vim-2)
-
VIM-7 metallo-beta-lactamase
-
beta-Lactamases
-
carbapenemase