SNIP1 is a candidate modifier of the transcriptional activity of c-Myc on E box-dependent target genes

Mol Cell. 2006 Dec 8;24(5):771-783. doi: 10.1016/j.molcel.2006.11.006.


Using a yeast two-hybrid screen, we found that SNIP1 (Smad nuclear-interacting protein 1) associates with c-Myc, a key regulator of cell proliferation and transformation. We demonstrate that SNIP1 functions as an important regulator of c-Myc activity, binding the N terminus of c-Myc through its own C terminus, and that SNIP1 enhances the transcriptional activity of c-Myc both by stabilizing it against proteosomal degradation and by bridging the c-Myc/p300 complex. These effects of SNIP1 on c-Myc likely contribute to synergistic effects of SNIP1, c-Myc, and H-Ras in inducing formation of foci in an in vitro transformation assay and also in supporting anchorage-independent growth. The significant association of SNIP1 and c-Myc staining in a non-small cell lung cancer tissue array is further evidence that their activities might be linked and suggests that SNIP1 might be an important modulator of c-Myc activity in carcinogenesis.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Cell Line
  • Cells, Cultured
  • Chromatin Immunoprecipitation
  • Humans
  • Immunohistochemistry
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Promoter Regions, Genetic / genetics*
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism*
  • RNA, Small Interfering / metabolism
  • RNA-Binding Proteins
  • Reverse Transcriptase Polymerase Chain Reaction
  • S-Phase Kinase-Associated Proteins / metabolism
  • Sensitivity and Specificity
  • Tissue Array Analysis
  • Transcription, Genetic*
  • Two-Hybrid System Techniques


  • Intracellular Signaling Peptides and Proteins
  • Proto-Oncogene Proteins c-myc
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • S-Phase Kinase-Associated Proteins
  • SNIP1 protein, human