Analysis of bacterial cultures in infectious keratitis, 1997 to 2004

Am J Ophthalmol. 2006 Dec;142(6):1066-8. doi: 10.1016/j.ajo.2006.06.056.


Purpose: To review bacterial culture results in infectious keratitis at a tertiary referral center.

Design: Retrospective case series.

Methods: Analysis of Gram stain, bacterial culture, and targeted antibiotic sensitivities of all cases of presumed infectious keratitis at Duke University Eye Center from 1997 to 2004 (n = 453).

Results: Cultures were positive in 307 cases (68%); 21% demonstrated polymicrobial growth. Among 388 isolates, 81% were Gram-positive. Gram stain results were available in 334 cases, demonstrating 18% sensitivity and 94% specificity. Antibiotic sensitivities revealed 24% resistance of tested S. aureus isolates to cefazolin, and <5% resistance of tested gram-negative isolates to various antibiotics, with no statistically significant increase from 1997 to 2004.

Conclusions: In this study, the microbial profile has remained stable from 1997 to 2004. Gram stain studies were of poor sensitivity, while culture yields were significantly higher and demonstrated a relatively high polymicrobial infection rate. Increasing antibiotic resistance was not clearly demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Microbial
  • Eye Infections, Bacterial / microbiology*
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Positive Bacteria / drug effects
  • Gram-Positive Bacteria / isolation & purification*
  • Humans
  • Keratitis / microbiology*
  • Microbial Sensitivity Tests
  • Retrospective Studies


  • Anti-Bacterial Agents