Applications of real-time PCR technology to mycobacterial research

Indian J Med Res. 2006 Oct;124(4):385-98.


Real-time polymerase chain reaction (PCR) is being used worldwide for various research purposes. In this review we discuss the principles of real-time PCR, different methodologies and chemistries available and their applications in mycobacterial research. This technology allows for the direct detection of PCR product during the exponential phase of the reaction. Being a very powerful, accurate, rapid and sensitive method, this technique holds immense promise for detection of mycobacteria, differentiation of mycobacterial species, quantification of mycobacterial load and detection of drug resistance in mycobacterial infection.

Publication types

  • Review

MeSH terms

  • Colony Count, Microbial
  • Drug Resistance, Bacterial / genetics
  • Humans
  • Mycobacterium / classification
  • Mycobacterium / drug effects
  • Mycobacterium / genetics*
  • Mycobacterium / isolation & purification
  • Mycobacterium Infections / diagnosis
  • Mycobacterium Infections / drug therapy
  • Mycobacterium Infections / microbiology
  • Mycobacterium tuberculosis / genetics
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / trends
  • Species Specificity