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Comparative Study
. 1991 Aug;228(1-2):201-8.
doi: 10.1007/BF00282466.

The Transposable Element Tam1 From Antirrhinum Majus Shows Structural Homology to the Maize Transposon En/Spm and Has No Sequence Specificity of Insertion

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Comparative Study

The Transposable Element Tam1 From Antirrhinum Majus Shows Structural Homology to the Maize Transposon En/Spm and Has No Sequence Specificity of Insertion

W K Nacken et al. Mol Gen Genet. .

Abstract

We present the genomic structure of Tam1, a transposable element from Antirrhinum majus. The Tam1 element is 15.2 kb long and includes two genes that are transcribed to produce a 2.4 kb (tnp1) and a 5 kb mRNA (tnp2). These transcripts partially overlap and the exons are scattered over the whole element. Tnp1 encodes a 53 kDa protein as deduced from the cDNA sequence. The 5 kb transcript of tnp2 contains an open reading frame that shares 45% homology with part of the tnpD gene of En/Spm from maize and 48% homology with an open reading frame of the Tgm element from Glycine max. We discuss the possible functions of these genes by analogy with En/Spm. Additionally, a number of flanking sequences of Tam1 insertions were analysed to investigate the sequence specificity of insertion. From these studies we conclude that Tam1 transposes predominantly into AT-rich regions that can be unique as well as repetitive. No specific target sequence of insertion could be found.

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