The transposable element Tam1 from Antirrhinum majus shows structural homology to the maize transposon En/Spm and has no sequence specificity of insertion

Mol Gen Genet. 1991 Aug;228(1-2):201-8. doi: 10.1007/BF00282466.


We present the genomic structure of Tam1, a transposable element from Antirrhinum majus. The Tam1 element is 15.2 kb long and includes two genes that are transcribed to produce a 2.4 kb (tnp1) and a 5 kb mRNA (tnp2). These transcripts partially overlap and the exons are scattered over the whole element. Tnp1 encodes a 53 kDa protein as deduced from the cDNA sequence. The 5 kb transcript of tnp2 contains an open reading frame that shares 45% homology with part of the tnpD gene of En/Spm from maize and 48% homology with an open reading frame of the Tgm element from Glycine max. We discuss the possible functions of these genes by analogy with En/Spm. Additionally, a number of flanking sequences of Tam1 insertions were analysed to investigate the sequence specificity of insertion. From these studies we conclude that Tam1 transposes predominantly into AT-rich regions that can be unique as well as repetitive. No specific target sequence of insertion could be found.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA / isolation & purification
  • DNA Transposable Elements / genetics*
  • Molecular Sequence Data
  • Plants / genetics*
  • Poly A / isolation & purification
  • Polymerase Chain Reaction
  • RNA / isolation & purification
  • RNA, Messenger
  • Sequence Homology, Nucleic Acid
  • Zea mays / genetics*


  • DNA Transposable Elements
  • RNA, Messenger
  • Poly A
  • RNA
  • DNA