Selective induction of B7/BB-1 on interferon-gamma stimulated monocytes: a potential mechanism for amplification of T cell activation through the CD28 pathway

Cell Immunol. 1991 Oct 15;137(2):429-37. doi: 10.1016/0008-8749(91)90091-o.


The B cell activation antigen B7/BB-1 is the natural ligand for the T cell antigen CD28 and these two molecules are capable of mediating T-B cell adhesion. Engagement of the CD28 pathway provides a costimulatory signal to T cells leading to enhanced lymphokine production. We report that interferon-gamma (INF-gamma) induces the expression of B7/BB-1 on monocytes. This induction was very specific since other cytokines and stimuli which activate monocytes including M-CSF, GM-CSF, IL3, TNF-alpha, and LPS were unable to induce B7/BB-1. Following culture of monocytes with INF-gamma, maximal mRNA and cell surface B7/BB-1 expression was detected at 12 and 24 hr, respectively. In addition to antigen presentation, optimal T cell activation and lymphokine synthesis require an additional cell to cell contact signal provided by the antigen presenting cell. The induction of B7/BB-1 on monocytes and subsequent heterophilic interaction of B7/BB-1 with CD28 may provide a mechanism for the amplification of T cell proliferation and lymphokine production by INF-gamma activated monocytes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD / metabolism*
  • Antigens, Differentiation, T-Lymphocyte / metabolism*
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism*
  • B7-1 Antigen
  • Blotting, Northern
  • CD28 Antigens
  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism
  • Cytokines / pharmacology
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / pharmacology*
  • Lymphocyte Activation
  • Monocytes / metabolism*
  • RNA, Messenger / genetics


  • Antigens, CD
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface
  • B7-1 Antigen
  • CD28 Antigens
  • Cell Adhesion Molecules
  • Cytokines
  • RNA, Messenger
  • Interferon-gamma