Objective: To explore whether propofol plays a protective role in kidney injury during acute respiratory distress syndrome (ARDS) by affecting the expression of Gq/11 protein.
Methods: An ARDS model in rats was reproduced by intravenous injection of oleic acid (OA). Healthy Wistar rats were randomly divided into pre-treatment group in which rats received propofol before given OA (P group), OA group with rats received propofol 90 minutes after iv OA (OA group) and control group (C group). Blood pressure, plasma and kidney homogenate contents of angiotensin-conversion enzyme (ACE), lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) were assessed during the experimental period, and the concentration of Gq/11 protein was determined by Western blot.
Results: The concentration of Gq/11 protein of P group [(124.68+/-19.38)%] was increased (24.68+/-19.38)% compared with C group [(100.00+/-0)%, P<0.01], and it was obviously decreased compared with OA group [(149.34+/-20.04)%, P<0.01]. ACE [(16.52+/-1.37) micromol x min(-1)xg(-1)], LDH activity [(1.20+/-0.16) kU/g] and MDA content [(1.51+/-0.35) micromol/g], respectively, in homogenate of P group were lower than OA group [(17.56+/-1.02) micromol x min(-1)xg(-1), (1.41+/-0.16) kU/g, (1.94+/-0.16) micromol/g, respectively, all P<0.01].
Conclusion: Pre-treatment with propofol can reduce the concentration of Gq/11 protein in kidney tissue, and it can ameliorate the injury to the kidney during ARDS.