Highly sensitive assay for the measurement of serotonin in microdialysates using capillary high-performance liquid chromatography with electrochemical detection

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):303-9. doi: 10.1016/j.jchromb.2006.11.045. Epub 2006 Dec 12.


A highly sensitive isocratic capillary high-performance liquid chromatographic (HPLC) method with electrochemical detection (ED) for the simultaneous measurement of serotonin (5-hydroxytryptamine, 5-HT) and its metabolite 5-hydroxyindole-3-acetic acid (5-HIAA) in microdialysates has been developed using a 0.5 mm i.d. capillary column and a 11-nL detection cell. This method, validated on both pharmacological and analytical bases, can be performed using injection volumes as low as 1 microL. The limits of detection were 5.6 x 10(-11)mol/L and 3.0 x 10(-9)mol/L for 5-HT and 5-HIAA. Several applications of the present method are given on microdialysates from rodent brain and human spinal cord.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Electrochemistry / methods*
  • Hydroxyindoleacetic Acid / analysis
  • Microdialysis
  • Sensitivity and Specificity
  • Serotonin / metabolism*


  • Serotonin
  • Hydroxyindoleacetic Acid