A comparison of probes for restriction fragment length polymorphism (RFLP) typing of Legionella pneumophila serogroup 1 strains

J Med Microbiol. 1991 Sep;35(3):152-8. doi: 10.1099/00222615-35-3-152.

Abstract

The use of probes derived from rRNA sequences to detect restriction fragment length polymorphisms (RFLPs) associated with the ribosomal RNA genes for epidemiological typing (ribotyping) is a powerful and readily applicable tool. Different probes and enzymes for ribotyping were compared for a series of 73 unrelated Legionella pneumophila serogroup 1 strains. The probes compared were cDNAs, transcribed from L. pneumophila or Escherichia coli rRNA subunits, and a cloned L. pneumophila rRNA gene. The cloned rRNA gene probe gave the best discrimination and this probe was further compared with cloned probes comprised of randomly selected (non-rRNA) parts of the L. pneumophila chromosome. In this instance the greatest discrimination was achieved when one of the non-ribosomal RNA gene probes was employed. The overall discrimination of RFLP typing was enhanced by combining the data obtained with both rRNA and non-rRNA probes.

Publication types

  • Comparative Study

MeSH terms

  • Bacteriophage lambda / genetics
  • Deoxyribonucleases, Type II Site-Specific
  • Legionella / classification*
  • Legionella / genetics
  • Phenotype
  • Polymorphism, Restriction Fragment Length*
  • RNA Probes*
  • RNA, Bacterial
  • RNA, Ribosomal
  • Serotyping* / methods

Substances

  • RNA Probes
  • RNA, Bacterial
  • RNA, Ribosomal
  • ATCGAT-specific type II deoxyribonucleases
  • CCSGG-specific type II deoxyribonucleases
  • Deoxyribonucleases, Type II Site-Specific