The association of VP1 unique region protein in acute parvovirus B19 infection and anti-phospholipid antibody production

Clin Chim Acta. 2007 Mar;378(1-2):59-65. doi: 10.1016/j.cca.2006.10.016. Epub 2006 Nov 10.


Background: Previous studies have postulated a connection between human parvovirus B19 (B19) infection and anti-phospholipid antibodies (aPL). Recently, the phospholipase domain of B19 has been linked to B19-VP1 unique region (VP1u). To elucidate the roles of VP1u in B19 infection and aPL production, the major reactivity of anti-B19-VP1u, anti-cardiolipin antibody (aCL), and anti-beta2-glycoprotein I (beta2GPI) antibody was evaluated.

Methods: Sera from 102 clinically suspected cases of B19 infection were analyzed by nested PCR and ELISA. Humoral responses of anti-B19-VP1u and anti-B19-VP1uD175A IgM/IgG antibodies, aCL and the anti-beta2GPI antibody were assessed by Western blot and ELISA. Absorption experiments were also performed to determine the binding specificity of immunoglobulins to B19-VP1u, CL and beta2GPI.

Results: Sera from patients with the diagnostic pattern DNA+/IgM+/IgG+ had a high frequency (57%) for recognition of CL and beta2GPI. Furthermore, adsorption experiments were performed by adding purified B19-VP1u, which partially suppressed the reactivity of anti-B19VP1u to CL and beta2GPI.

Conclusions: Serum from patients with acute B19 infection has a high frequency in recognition of CL and beta2GPI, and the phospholipase domain observed in the B19-VP1u may have contributed to the production of aPL. These findings may provide a clue for understanding the roles of B19-VP1u in B19 infection and aPL production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Antibodies, Antiphospholipid / biosynthesis*
  • Antibodies, Viral / blood
  • Capsid Proteins / genetics
  • Capsid Proteins / immunology*
  • Cardiolipins / blood
  • Child
  • DNA, Viral / blood
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Male
  • Middle Aged
  • Parvoviridae Infections / immunology*
  • Parvovirus B19, Human / immunology*
  • beta 2-Glycoprotein I / immunology


  • Antibodies, Antiphospholipid
  • Antibodies, Viral
  • Capsid Proteins
  • Cardiolipins
  • DNA, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • beta 2-Glycoprotein I
  • capsid protein VP1, parvovirus B19