Oncostatin M stimulates expression of stromal-derived factor-1 in human mesenchymal stem cells

Int J Biochem Cell Biol. 2007;39(3):650-9. doi: 10.1016/j.biocel.2006.11.003. Epub 2006 Nov 23.


Stromal-derived factor-1 (SDF-1) is a CXC chemokine that attracts leukocytes and endothelial progenitor cells. In the present study, we demonstrated that oncostatin M (OSM) stimulates expression and secretion of SDF-1 in both human adipose tissue-derived mesenchymal stem cells (hATSCs) and bone marrow-derived mesenchymal stem cells. The OSM-stimulated expression of SDF-1 in hATSCs was completely abrogated by pretreatment of the cells with U0126, an MEK-specific inhibitor, but not with AG490, a JAK2 inhibitor, or WHI-P131, a JAK3 inhibitor, suggesting that ERK, but not JAK2 and JAK3, is involved in the OSM-induced expression of SDF-1. Pretreatment of hATSCs with anti-VEGF neutralizing antibody or VEGF receptor inhibitors, SU5416 and KRN633, had no significant impact on the OSM induction of SDF-1. Furthermore, treatment of hATSCs with recombinant human VEGF165 or adenoviral overexpression of VEGF did not increase the expression of SDF-1. These results suggest that OSM induces secretion of SDF-1 through ERK-, but not VEGF-, dependent signaling pathways in mesenchymal stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology
  • Adipose Tissue / metabolism
  • Base Sequence
  • Butadienes / pharmacology
  • Cells, Cultured
  • Chemokine CXCL12
  • Chemokines, CXC / biosynthesis*
  • Chemokines, CXC / genetics*
  • DNA Primers / genetics
  • Gene Expression / drug effects
  • Humans
  • MAP Kinase Signaling System / drug effects
  • Mesenchymal Stem Cells / drug effects*
  • Mesenchymal Stem Cells / metabolism*
  • Nitriles / pharmacology
  • Oncostatin M / pharmacology*
  • Recombinant Proteins / pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Vascular Endothelial Growth Factor A / biosynthesis


  • Butadienes
  • CXCL12 protein, human
  • Chemokine CXCL12
  • Chemokines, CXC
  • DNA Primers
  • Nitriles
  • OSM protein, human
  • Recombinant Proteins
  • U 0126
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A
  • Oncostatin M