Inhibition of pulmonary metastasis of melanoma b16fo cells in C57BL/6 mice by a nutrient mixture consisting of ascorbic Acid, lysine, proline, arginine, and green tea extract

Exp Lung Res. Nov-Dec 2006;32(10):517-30. doi: 10.1080/01902140601098552.

Abstract

The authors investigated the effect of a nutrient mixture (NM) on lung metastasis by B16F0 melanoma cells in C57BL/6 female mice. Mice were divided into equal groups (1 to 6) and injected via tail vein with B16F0 cells (groups 1 to 4), B16FO cells pretreated with NM (group 5), or saline (group 6). Groups 1, 3, 4, 5, and 6 were fed the control diet and group 2 the 0.5% NM supplemented diet. Groups 3 and 4 received NM intraperitoneally (IP) and intravenously (IV), respectively. Two weeks later, pulmonary metastatic colonies were counted. Pulmonary colonization was reduced by 63% in mice supplemented with NM diet, by 86% in mice receiving NM by IP and IV injections, and completely inhibited in mice injected with melanoma cells pretreated with NM. These results show that NM is effective in inhibiting the metastasis of B16FO melanoma cells.

MeSH terms

  • Animals
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology*
  • Arginine / pharmacology
  • Ascorbic Acid / pharmacology
  • Camellia sinensis
  • Dietary Supplements*
  • Drug Screening Assays, Antitumor
  • Female
  • Lung / drug effects
  • Lung / pathology
  • Lung Neoplasms / drug therapy*
  • Lung Neoplasms / secondary
  • Lysine / pharmacology
  • Melanoma / drug therapy*
  • Melanoma / pathology
  • Mice
  • Mice, Inbred C57BL
  • Neoplasm Metastasis / drug therapy*
  • Neoplasm Metastasis / pathology
  • Plant Extracts / pharmacology
  • Proline / pharmacology
  • Skin Neoplasms / drug therapy*
  • Skin Neoplasms / pathology

Substances

  • Plant Extracts
  • green tea extract AR25
  • Arginine
  • Proline
  • Lysine
  • Ascorbic Acid