Inactivation of human immunodeficiency virus type 1 by ozone in vitro

Blood. 1991 Oct 1;78(7):1882-90.


A device was designed to deliver a constant source of given concentrations of ozone to fluids containing human immunodeficiency virus type 1 (HIV-1). Ozone was found to inactivate HIV-1 virions in a dose-dependent manner. Greater than 11 log inactivation was achieved within 2 hours at a concentration of 1,200 ppm ozone. Similar concentrations of ozone had minimal effect on factor VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation, and/or a perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as a means to inactivate human retroviruses in human body fluids and blood product preparations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antiviral Agents / administration & dosage
  • Antiviral Agents / pharmacology*
  • Body Fluids / microbiology
  • Cell Line
  • Dose-Response Relationship, Drug
  • Factor VIII / metabolism
  • HIV Core Protein p24 / analysis
  • HIV Core Protein p24 / metabolism
  • HIV Reverse Transcriptase
  • HIV-1 / drug effects*
  • HIV-1 / physiology
  • Humans
  • Ozone / administration & dosage
  • Ozone / pharmacology*
  • Reverse Transcriptase Inhibitors


  • Antiviral Agents
  • HIV Core Protein p24
  • Reverse Transcriptase Inhibitors
  • Ozone
  • Factor VIII
  • HIV Reverse Transcriptase