Repression of integrin beta 1 subunit expression by antisense RNA

Cell Struct Funct. 1991 Jun;16(3):241-9. doi: 10.1247/csf.16.241.

Abstract

A quail cell line (QT6-c) was co-transfected with pTEX vector expressing RNA complementary to chicken integrin beta 1 subunit mRNA (Anti-Int) and pRSVneo vector by a calcium phosphate method. Transfectants showing reduced expression of quail integrin beta 1 subunit were selected with an immunoblot assay, and a few positive clones were examined in detail. Northern blot and immunoblot analyses revealed that the Anti-Int caused a clear reduction of the transcript encoding integrin beta 1 subunit depending on culture conditions. The number of cell surface integrins also decreased in proportion to the decrement of the total amount of integrin beta 1 subunits. When one transfectant (QA23) was cultured in a serum-free medium, cell shape changed from fibroblast-like to neuron-like morphology accompanied by a low growth rate, and the cells did not form focal contact on fibronectin. A similar morphological change occurred in QT6-c cells when the cells were infected with Rous Sarcoma virus, which could produce the Anti-Int. The QA23 cells did not attach to fibronectin as efficiently as did the original QT6-c cells. These data suggest that reduced expression of integrin beta 1 subunit affects cell growth as well as cell morphology by disordering the interaction between integrins and matrix proteins and/or cytoplasmic proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Division / drug effects
  • Cells, Cultured
  • Culture Media, Serum-Free
  • Fibroblasts / cytology
  • Fibroblasts / metabolism
  • Fibronectins / metabolism
  • Gene Expression / genetics*
  • Genetic Vectors
  • Integrins / genetics*
  • Integrins / metabolism
  • Macromolecular Substances
  • Precipitin Tests
  • Quail
  • RNA / genetics
  • RNA / metabolism
  • RNA, Antisense / genetics*
  • RNA, Antisense / metabolism
  • Transfection

Substances

  • Culture Media, Serum-Free
  • Fibronectins
  • Integrins
  • Macromolecular Substances
  • RNA, Antisense
  • RNA