The germinal center response is impaired in the absence of T cell-expressed CXCR5

Eur J Immunol. 2007 Jan;37(1):100-9. doi: 10.1002/eji.200636486.


Germinal centers support the differentiation of memory B cells and long-lived antibody-secreting cells during infection or upon vaccination. Here, we constructed mice with T cells that selectively lack the chemokine receptor CXCR5 to determine if expression of this receptor by T cells is mandatory for germinal center formation and function. In these animals, germinal centers that are properly localized in B cell follicles and contain T cells do form after immunization with a thymus-dependent antigen. However, fewer and smaller germinal centers form, resulting in a significant reduction in the frequency of germinal center B cells. The defect in germinal center formation is paralleled by decreased frequencies of isotype-switched antibody-secreting cells in the spleen and bone marrow and reduced serum concentrations of total and high-affinity hapten-specific IgG1. The results demonstrate that although CXCR5-dependent T cell positioning is important for maximal induction and expansion of germinal centers, stimulation of isotype class switching, and development of antibody-secreting cells that seed the spleen and bone marrow, it is not absolutely required for the formation and function of follicular germinal centers.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / immunology
  • B-Lymphocyte Subsets / metabolism
  • Bone Marrow Cells / immunology
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology
  • Chemotaxis, Leukocyte / genetics
  • Chemotaxis, Leukocyte / immunology
  • Crosses, Genetic
  • Germinal Center / immunology*
  • Germinal Center / metabolism*
  • Germinal Center / pathology
  • Immunoglobulin Class Switching / genetics
  • Immunoglobulin Isotypes / biosynthesis
  • Immunoglobulin Isotypes / genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Radiation Chimera
  • Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, CXCR5
  • Receptors, Chemokine / biosynthesis*
  • Receptors, Chemokine / deficiency*
  • Receptors, Chemokine / physiology
  • Spleen / cytology
  • Spleen / immunology
  • Spleen / metabolism
  • T-Lymphocyte Subsets / immunology*
  • T-Lymphocyte Subsets / metabolism*
  • T-Lymphocyte Subsets / pathology


  • CXCR5 protein, mouse
  • Immunoglobulin Isotypes
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, CXCR5
  • Receptors, Chemokine