Blockade of high mobility group box-1 protein attenuates experimental severe acute pancreatitis

World J Gastroenterol. 2006 Dec 21;12(47):7666-70. doi: 10.3748/wjg.v12.i47.7666.


Aim: To examine the effects of anti-high mobility group box 1 (HMGB1) neutralizing antibody in experimental severe acute pancreatitis (SAP).

Methods: SAP was induced by creating closed duodenal loop in C3H/HeN mice. SAP was induced immediately after intraperitoneal injection of anti-HMGB1 neutralizing antibody (200 microg). Severity of pancreatitis, organ injury (liver, kidney and lung), and bacterial translocation to pancreas was examined 12 h after induction of SAP.

Results: Anti-HMGB1 neutralizing antibody significantly improved the elevation of the serum amylase level and the histological alterations of pancreas and lung in SAP. Anti-HMGB1 antibody also significantly ameliorated the elevations of serum alanine aminotransferase and creatinine in SAP. However, anti-HMGB1 antibody worsened the bacterial translocation to pancreas.

Conclusion: Blockade of HMGB1 attenuated the development of SAP and associated organ dysfunction, suggesting that HMGB1 may act as a key mediator for inflammatory response and organ injury in SAP.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology*
  • Female
  • HMGB1 Protein / antagonists & inhibitors*
  • HMGB1 Protein / immunology*
  • Immunotherapy / methods*
  • Mice
  • Mice, Inbred C3H
  • Pancreatitis / immunology
  • Pancreatitis / pathology
  • Pancreatitis / therapy*
  • Severity of Illness Index


  • Antibodies
  • HMGB1 Protein