Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells

Methods Mol Biol. 2007;360:155-61. doi: 10.1385/1-59745-165-7:155.


In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

MeSH terms

  • Image Processing, Computer-Assisted
  • Microscopy / instrumentation*
  • Microscopy / methods*
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Small Interfering*
  • Surface Properties
  • Transfection


  • RNA, Small Interfering