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, 364, 43-62

First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction

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First Analysis of Macromolecular Crystals: Biochemistry and X-Ray Diffraction

David Jeruzalmi. Methods Mol Biol.

Abstract

Methods are described for performing a first analysis of newly prepared macromolecular crystals. Biochemical analysis using denaturing gel electrophoresis on dissolved crystals informs on the content of the crystal. Detailed protocols are given for mounting crystals in capillaries or fiber loops in preparation for analysis of their X-ray diffractive properties.

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