Antibodies to T-cell Ig and mucin domain-containing proteins (Tim)-1 and -3 suppress the induction and progression of murine allergic conjunctivitis

Biochem Biophys Res Commun. 2007 Feb 2;353(1):211-6. doi: 10.1016/j.bbrc.2006.12.023. Epub 2006 Dec 11.


The T cell Ig and mucin domain-containing proteins (Tim) regulate Th1- and Th2-mediated immune responses. We investigated the ability of Abs blocking Tim-1 or Tim-3 ligand-binding activity to prevent and treat murine experimental allergic conjunctivitis (EC), a Th2-mediated disease. Treatment with either Ab during the induction phase of EC in actively immunized wild-type mice suppressed EC and upregulated Th1 and Th2 immune responses. In contrast, both Abs exacerbated EC in actively immunized IFN-gamma-knockout mice. Thus, both anti-Tim Abs suppress the pathogenic immune responses generated in the induction phase by upregulating systemic IFN-gamma production. Treatment of actively immunized mice and passively immunized mice with either anti-Tim Ab just prior to RW challenge also suppressed EC. Thus, treatment with anti-Tim-1 or anti-Tim-3 Ab can suppress both the induction and progression of EC, which could indicate potential preventive and/or therapeutic approaches for allergic diseases such as allergic conjunctivitis.

MeSH terms

  • Animals
  • Antibodies / immunology
  • Antibodies / therapeutic use*
  • Conjunctivitis, Allergic / immunology*
  • Conjunctivitis, Allergic / prevention & control
  • Hepatitis A Virus Cellular Receptor 2
  • Immunoglobulin G / immunology*
  • Immunotherapy, Adoptive / methods
  • Membrane Proteins / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Protein Structure, Tertiary
  • Receptors, Virus / immunology*
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / transplantation*


  • Antibodies
  • Havcr2 protein, mouse
  • Hepatitis A Virus Cellular Receptor 2
  • Immunoglobulin G
  • Membrane Proteins
  • Receptors, Virus
  • Timd2 protein, mouse