FT-IR imaging of native and tissue-engineered bone and cartilage

Biomaterials. 2007 May;28(15):2465-78. doi: 10.1016/j.biomaterials.2006.11.043. Epub 2006 Dec 18.


Fourier transform infrared (FT-IR) imaging and microspectroscopy have been extensively applied to the analyses of tissues in health and disease. Spatially resolved mid-IR data has provided insights into molecular changes that occur in diseases of connective or collagen-based tissues, including, osteoporosis, osteogenesis imperfecta, osteopetrosis and pathologic calcifications. These techniques have also been used to probe chemical changes associated with load, disuse, and micro-damage in bone, and with degradation and repair in cartilage. This review summarizes the applications of FT-IR microscopy and imaging for analyses of bone and cartilage in healthy and diseased tissues, and illustrates the application of these techniques for the characterization of tissue-engineered bone and cartilage.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Bone and Bones / anatomy & histology
  • Bone and Bones / chemistry*
  • Cartilage / anatomy & histology
  • Cartilage / chemistry*
  • Collagen / analysis
  • Diagnostic Imaging / methods*
  • Humans
  • Proteoglycans / analysis
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Tissue Engineering / methods


  • Proteoglycans
  • Collagen