Identification of a cDNA clone specific for the oligodendrocyte-derived repulsive extracellular matrix molecule J1-160/180

J Neurosci Res. 1991 Jul;29(3):299-307. doi: 10.1002/jnr.490290305.

Abstract

A cDNA clone specific for the oligodendrocyte-derived extracellular matrix glycoproteins J1-160/180 was obtained from a lambda ZAPII expression library using polyclonal antibodies generated against mouse J1-160. The library was constructed from poly(A)(+)-RNA isolated from O1 antigen-positive rat oligodendrocytes. The cDNA clone expressed a fusion protein that was recognized by the J1-160/180-specific monoclonal antibodies 596, 619, and 620, and, weakly, 597. The fusion protein was not recognized by polyclonal antibodies to mouse J1/tenascin. The cDNA clone with an insert of approximately 5.6 kb in size contained the nucleotide sequence coding for the amino acid sequence of the N-terminus of a tryptic peptide derived from mouse J1-160. The developmental and tissue distribution of the mRNA recognized by the cDNA clone is in agreement with the described expression of the J1-160/180 proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal
  • Base Sequence
  • Blotting, Southern
  • Blotting, Western
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Cloning, Molecular
  • DNA / genetics
  • Extracellular Matrix Proteins / analysis
  • Extracellular Matrix Proteins / genetics*
  • Extracellular Matrix Proteins / physiology
  • Gene Library
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Oligodendroglia / physiology*
  • Poly A / genetics
  • Poly A / isolation & purification
  • RNA / genetics
  • RNA / isolation & purification
  • RNA, Messenger
  • Rats
  • Recombinant Fusion Proteins / analysis
  • Restriction Mapping
  • Tenascin

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules, Neuronal
  • Extracellular Matrix Proteins
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Tenascin
  • tenascin R
  • Poly A
  • RNA
  • DNA