Tvp38, Tvp23, Tvp18 and Tvp15: novel membrane proteins in the Tlg2-containing Golgi/endosome compartments of Saccharomyces cerevisiae

Exp Cell Res. 2007 Feb 15;313(4):688-97. doi: 10.1016/j.yexcr.2006.11.008. Epub 2006 Nov 22.


Four previously uncharacterized proteins (Tvp38, Tvp23, Tvp18 and Tvp15) were found in Tlg2-containing membrane by proteomic analysis of immunoisolated Golgi subcompartments of Saccharomyces cerevisiae (Inadome et al., Mol. Cell. Biol., 25 (2005) 7696-7710). Immunofluorescence double staining of HA-tagged Tvp proteins and myc-tagged tSNAREs supported that these proteins mainly localize in the Tlg2-containing compartments. Conserved sequences of Tvp38, Tvp23 and Tvp18 are found in higher eukaryotes, but these homologues have not been characterized yet. All Tvp proteins were nonessential for growth under laboratory conditions. Immunoprecipitation of Tvp proteins indicated that Tvp23, Tvp18 and Tvp15 are in an interactive network with Yip1-family proteins, Yip4 and Yip5. They may collectively assist in the effective maintenance/function of the late Golgi/endosomal compartments. Disruptions of tvp15 and tvp23 showed synthetic aggravation with ypt6 or ric1 null mutation. Processing of carboxypeptidase Y and alkaline phosphatase in tvp disruptants occurred as in the wild type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • Endosomes / metabolism*
  • Golgi Apparatus / metabolism*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism
  • Membrane Proteins / physiology*
  • Molecular Sequence Data
  • Mutant Proteins / physiology
  • Protein Interaction Mapping
  • Qa-SNARE Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins / physiology
  • Saccharomyces cerevisiae*
  • Sequence Homology, Amino Acid
  • Vesicular Transport Proteins / chemistry
  • Vesicular Transport Proteins / metabolism
  • Vesicular Transport Proteins / physiology


  • Adaptor Proteins, Signal Transducing
  • Carrier Proteins
  • Membrane Proteins
  • Mutant Proteins
  • Qa-SNARE Proteins
  • Saccharomyces cerevisiae Proteins
  • TLG2 protein, S cerevisiae
  • Vesicular Transport Proteins
  • Yip4 protein, S cerevisiae
  • Yip5 protein, S cerevisiae