Molecular cloning, structural characterization and functional expression of the human substance P receptor

Biochem Biophys Res Commun. 1991 Sep 30;179(3):1232-40. doi: 10.1016/0006-291x(91)91704-g.


A cDNA encoding the human substance P receptor (SPR) was isolated and the primary structure of the protein was deduced by nucleotide sequence analysis. This SPR consists of 407 residues and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SPR sequences demonstrated a 94.5% identity. The receptor was expressed in a COS-7 cell line and displayed a Kd for Tyr-1-SP binding of 0.24 nM. Ligand displacement by naturally occurring tachykinin peptides was SP much greater than neurokinin A greater than neurokinin B. SP stimulation of transfected cells resulted in a rapid and transient inositol 1,4,5-trisphosphate response. RNA blot hybridization and solution hybridization demonstrated that SPR mRNA was about 4.5 Kb in size, and was expressed in IM-9 lymphoblast and U373-MG astrocytoma cells, as well as in spinal cord and lung but not in liver.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • Cell Line
  • Cloning, Molecular / methods
  • DNA / genetics
  • DNA / isolation & purification
  • Exons
  • Humans
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Kinetics
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • RNA, Messenger / genetics
  • Rats
  • Receptors, Neurokinin-1
  • Receptors, Neurotransmitter / genetics*
  • Receptors, Neurotransmitter / metabolism
  • Recombinant Proteins / metabolism
  • Sequence Homology, Nucleic Acid
  • Substance P / metabolism
  • Transfection


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, Neurokinin-1
  • Receptors, Neurotransmitter
  • Recombinant Proteins
  • Substance P
  • Inositol 1,4,5-Trisphosphate
  • DNA

Associated data

  • GENBANK/M74290
  • GENBANK/M74785
  • GENBANK/M74786
  • GENBANK/M74787
  • GENBANK/M74788
  • GENBANK/M74790
  • GENBANK/M81108
  • GENBANK/M81109
  • GENBANK/S57525
  • GENBANK/S57527