Isolation of Yersinia-specific T cell clones from the synovial membrane and synovial fluid of a patient with reactive arthritis

Arthritis Rheum. 1991 Sep;34(9):1151-7. doi: 10.1002/art.1780340911.


Synovial fluid (SF) mononuclear cells from patients with reactive arthritis (ReA) proliferate in vitro when challenged with ReA-associated bacteria, the maximal response being for the organism causing the triggering infection. We report the results of a study of the antigenic specificity of synovial T lymphocytes from an HLA-B27 positive ReA patient whose SF mononuclear cells responded preferentially to Yersinia antigens. This is the first report of the isolation of Yersinia-specific T cell clones from synovial membrane (obtained by closed-needle synovial biopsy). We present a detailed analysis of these clones, together with others obtained from the SF.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Bacterial / immunology
  • Arthritis, Infectious / blood
  • Arthritis, Infectious / pathology*
  • Arthritis, Reactive / microbiology
  • Arthritis, Reactive / pathology*
  • Biopsy, Needle
  • Cell Separation
  • Epitopes
  • HLA-B27 Antigen / immunology
  • Histocompatibility Antigens Class II / immunology
  • Humans
  • Male
  • Phenotype
  • Synovial Fluid / cytology*
  • Synovial Fluid / microbiology
  • Synovial Membrane / microbiology
  • Synovial Membrane / pathology*
  • T-Lymphocytes / microbiology
  • T-Lymphocytes / pathology*
  • Yersinia / immunology*
  • Yersinia / isolation & purification
  • Yersinia Infections / microbiology
  • Yersinia Infections / pathology*


  • Antibodies, Bacterial
  • Epitopes
  • HLA-B27 Antigen
  • Histocompatibility Antigens Class II