Rapid, small-scale RNA isolation from tissue culture cells

Biotechniques. 1991 Sep;11(3):324, 326-7.


A rapid and simple protocol for the isolation of RNA from transfected tissue culture cells is described. The protocol employs a guanidinium thiocyanate/phenol mixture to lyse cells directly from tissue culture plates and extract the total RNA. A total of six simple steps, which can be accomplished within 2.5 hours, are required. The protocol reproducibly yields 20-40 micrograms RNA from 0.5 x 10(6) - 1 x 10(6) cells per sample. The quality of the RNA obtained is sufficient for reverse transcriptase assays such as oligonucleotide-directed primer extension and random-primed cDNA synthesis.

MeSH terms

  • Animals
  • CHO Cells
  • Cricetinae
  • Genetic Techniques
  • RNA / isolation & purification*
  • Rats
  • Time Factors
  • Transfection


  • RNA