Nitric oxide synthase protein and mRNA are discretely localized in neuronal populations of the mammalian CNS together with NADPH diaphorase

Neuron. 1991 Oct;7(4):615-24. doi: 10.1016/0896-6273(91)90374-9.


Nitric oxide is a free radical that has been recently recognized as a neural messenger molecule. Nitric oxide synthase has now been purified and molecularly cloned from brain. Using specific antibodies and oligonucleotide probes, we have localized brain nitric oxide synthase to discrete neuronal populations in the rat and primate brain. Nitric oxide synthase is exclusively neuronal, and its localization is absolutely coincident with NADPH diaphorase staining in both rat and primate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Oxidoreductases / genetics
  • Amino Acid Oxidoreductases / metabolism*
  • Animals
  • Blotting, Western
  • Brain / metabolism*
  • Haplorhini
  • Immunohistochemistry
  • Male
  • NADPH Dehydrogenase / metabolism*
  • Neurons / metabolism*
  • Nitric Oxide Synthase
  • Nucleic Acid Hybridization
  • Precipitin Tests
  • RNA, Messenger / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Staining and Labeling
  • Tissue Distribution


  • RNA, Messenger
  • Nitric Oxide Synthase
  • Amino Acid Oxidoreductases
  • NADPH Dehydrogenase