Vascular cell adhesion molecule-1 and eosinophil adhesion to cultured human umbilical vein endothelial cells in vitro

Am J Respir Cell Mol Biol. 1991 Nov;5(5):445-50. doi: 10.1165/ajrcmb/5.5.445.

Abstract

Cultured human umbilical vein endothelial cell (EC) monolayers stimulated with 10 ng/ml tumor necrosis factor demonstrate a time-dependent increase in the expression of the vascular cell adhesion molecule-1 (VCAM-1) with maintained maximal expression at 24 h following EC activation. A monoclonal antibody (mAb) directed against VCAM-1 (1G11) significantly inhibited the adhesion of eosinophils, but not neutrophils, to EC, which had been activated by tumor necrosis factor-alpha for 24 h, but only when eosinophils had been pretreated with an mAb directed against the common beta chain of the CD11/CD18 complex. In the absence of pretreatment with anti-CD18, mAb 1G11 had no significant effect on eosinophil adhesion. These results suggest that eosinophils bind to VCAM-1. However, the functional capacity in this model of the eosinophil receptor for VCAM-1 is likely to be minor compared with the activity of the CD11/CD18 leukocyte adhesion molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion
  • Cell Adhesion Molecules / metabolism*
  • Cells, Cultured
  • E-Selectin
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Eosinophils / cytology
  • Eosinophils / metabolism*
  • Humans
  • Neutrophils / cytology
  • Neutrophils / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology
  • Umbilical Veins
  • Vascular Cell Adhesion Molecule-1

Substances

  • Cell Adhesion Molecules
  • E-Selectin
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1