Relationship of the chemical structure and immunobiological activities of lipoteichoic acid from Streptococcus faecalis (Enterococcus hirae) ATCC 9790

FEMS Microbiol Immunol. 1991 Aug;3(4):211-8. doi: 10.1111/j.1574-6968.1991.tb04217.x.


Two molecular species of lipoteichoic acid (LTA 1 and LTA 2) were isolated from whole cells of Streptococcus faecalis (Enterococcus hirae) ATCC 9790 by hydrophobic chromatography on Octyl Sepharose CL-4B. Chemical analysis revealed that LTA 1 and LTA 2 contained two and four acyl lipid anchors respectively. LTA 1 was less active than LTA 2 in inducing cytokines, except interleukin-1 (IL-1), but their in vivo antitumour effects were similar. LTA 2 was a potent inducer of tumour necrosis factor (TNF) and interferon (IFN) production and had excellent antitumour activity against Meth A fibrosarcoma established in mice. Deacylation of LTA 2 by alkaline hydrolysis abolished these biological activities. The phosphatidylglycolipid fraction derived from LTA 2 after acid hydrolysis could also induce TNF, IFN, and IL-1 production, as well as having antitumour activity against Meth A fibrosarcoma. Therefore, the lipid anchor portion of S. faecalis LTA may play an important role in the manifestation of these various biological activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enterococcus faecalis / chemistry*
  • Female
  • Fibrosarcoma / drug therapy
  • Interferons / biosynthesis
  • Interleukin-1 / biosynthesis
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred ICR
  • Structure-Activity Relationship
  • Teichoic Acids / chemistry
  • Teichoic Acids / immunology
  • Teichoic Acids / pharmacology*
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Interleukin-1
  • Lipopolysaccharides
  • Teichoic Acids
  • Tumor Necrosis Factor-alpha
  • lipoteichoic acid
  • Interferons