Mesenchymal stem cell-mediated functional tooth regeneration in swine

PLoS One. 2006 Dec 20;1(1):e79. doi: 10.1371/journal.pone.0000079.


Mesenchymal stem cell-mediated tissue regeneration is a promising approach for regenerative medicine for a wide range of applications. Here we report a new population of stem cells isolated from the root apical papilla of human teeth (SCAP, stem cells from apical papilla). Using a minipig model, we transplanted both human SCAP and periodontal ligament stem cells (PDLSCs) to generate a root/periodontal complex capable of supporting a porcelain crown, resulting in normal tooth function. This work integrates a stem cell-mediated tissue regeneration strategy, engineered materials for structure, and current dental crown technologies. This hybridized tissue engineering approach led to recovery of tooth strength and appearance.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dental Papilla / cytology
  • Dental Papilla / physiology
  • Humans
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology*
  • Mice
  • Periodontal Ligament / cytology
  • Periodontal Ligament / physiology
  • Regeneration / physiology*
  • Swine
  • Swine, Miniature
  • Tooth / cytology
  • Tooth / physiology*
  • Tooth Root / cytology
  • Tooth Root / physiology
  • Transplantation, Heterologous