MALDI-MS-based imaging of small molecules and proteins in tissues

Curr Opin Chem Biol. 2007 Feb;11(1):29-35. doi: 10.1016/j.cbpa.2006.11.035. Epub 2006 Dec 20.


The direct analysis of tissues using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) enables both endogenous and exogenous compounds present in tissues to be detected with molecular specificity while maintaining their spatial orientation. This unique combination, coupled with excellent sensitivity and rapid analysis time, presents many potential advantages to a wide range of applications in diverse biological fields. Recent advances have shown how the technique can be applied to cancer research, neuroscience and pharmaceutical development. Examples include the use of unique protein profiles to classify human tumor tissues and predict patient outcomes, the discovery of protein changes in mouse cerebellum as a function of development, and the two-dimensional visualization of the distribution of a drug and first-pass metabolites in rat whole-body sections.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Cerebellum / chemistry
  • Cerebellum / metabolism
  • Cerebellum / pathology
  • Humans
  • Mice
  • Neoplasms / chemistry
  • Neoplasms / metabolism
  • Neoplasms / pathology
  • Neurosciences / methods
  • Pharmaceutical Preparations / analysis
  • Pharmaceutical Preparations / chemistry
  • Proteins* / analysis
  • Proteins* / chemistry
  • Proteins* / metabolism
  • Rats
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / trends
  • Technology, Pharmaceutical / methods
  • Tissue Distribution
  • Tissue Extracts / analysis*
  • Tissue Extracts / chemistry
  • Tissue Extracts / metabolism


  • Biomarkers
  • Pharmaceutical Preparations
  • Proteins
  • Tissue Extracts