Sustained activation of proton channels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not cPLA2 alpha activity

J Physiol. 2007 Mar 1;579(Pt 2):327-44. doi: 10.1113/jphysiol.2006.124248. Epub 2006 Dec 21.


The prevailing hypothesis that a signalling pathway involving cPLA(2)alpha is required to enhance the gating of the voltage-gated proton channel associated with NADPH oxidase was tested in human eosinophils and murine granulocytes. This hypothesis invokes arachidonic acid (AA) liberated by cPLA(2)alpha as a final activator of proton channels. In human eosinophils studied in the perforated-patch configuration, phorbol myristate acetate (PMA) stimulation elicited NADPH oxidase-generated electron current (I(e)) and enhanced proton channel gating identically in the presence or absence of three specific cPLA(2)alpha inhibitors, Wyeth-1, pyrrolidine-2 and AACOCF(3) (arachidonyl trifluoromethyl ketone). In contrast, PKC inhibitors GFX (GF109203X) or staurosporine prevented the activation of either proton channels or NADPH oxidase. PKC inhibition during the respiratory burst reversed the activation of both molecules, suggesting that ongoing phosphorylation is required. This effect of GFX was inhibited by okadaic acid, implicating phosphatases in proton channel deactivation. Proton channel activation by AA was partially reversed by GFX or staurosporine, indicating that AA effects are due in part to activation of PKC. In granulocytes from mice with the cPLA(2)alpha gene disrupted (knockout mice), PMA or fMetLeuPhe activated NADPH oxidase and proton channels in a manner indistinguishable from the responses of control cells. Thus, cPLA(2)alpha is not essential to activate the proton conductance or for a normal respiratory burst. Instead, phosphorylation of the proton channel or an activating molecule converts the channel to its activated gating mode. The existing paradigm for regulation of the concerted activity of proton channels and NADPH oxidase must be revised.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Arachidonic Acids / pharmacology
  • Carcinogens / pharmacology
  • Enzyme Activation / physiology
  • Enzyme Inhibitors / pharmacology
  • Eosinophils / enzymology*
  • Granulocytes / enzymology*
  • Group IV Phospholipases A2
  • Humans
  • Indoles / pharmacology
  • Ion Channels / drug effects
  • Ion Channels / physiology*
  • Maleimides / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, SCID
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • NADPH Oxidases / drug effects
  • NADPH Oxidases / physiology*
  • Okadaic Acid / pharmacology
  • Phospholipases A / antagonists & inhibitors
  • Phospholipases A / genetics
  • Phospholipases A / physiology*
  • Protein Kinase C / antagonists & inhibitors
  • Protein Kinase C / metabolism*
  • Protons*
  • Signal Transduction / physiology
  • Tetradecanoylphorbol Acetate / pharmacology


  • Arachidonic Acids
  • Carcinogens
  • Enzyme Inhibitors
  • Indoles
  • Ion Channels
  • Maleimides
  • Protons
  • Okadaic Acid
  • N-Formylmethionine Leucyl-Phenylalanine
  • NADPH Oxidases
  • Protein Kinase C
  • Phospholipases A
  • Group IV Phospholipases A2
  • bisindolylmaleimide I
  • Tetradecanoylphorbol Acetate