Sustained-release erythropoietin ameliorates cardiac function in infarcted rat-heart without inducing polycythemia

Circ J. 2007 Jan;71(1):132-7. doi: 10.1253/circj.71.132.


Background: The usefulness of sustained-release erythropoietin for improving left ventricular (LV) function without polycythemia was evaluated in a rat chronic myocardial infarction model.

Methods and results: Four weeks after left coronary artery ligation, 50 Sprague-Dawley rats were assigned to 5 groups (n=10, each). Control group had a gelatin sheet (20x20 mm) containing saline applied to the infarct area, whereas the 4 treatment groups had gelatin sheets incorporating erythropoietin 0.1 U, 1 U, 10 U and 100 U, respectively. Endpoint measurements performed at 8 weeks after the coronary ligation revealed that the fractional area change was larger for erythropoietin 1 U and 10 U than in the other 3 groups. The LV end-systolic elastance and the time constant of isovolumic relaxation were better for erythropoietin 1 U and 10 U than in the other 3 groups. The density of vessels larger than 50 microm in diameter was the highest in the erythropoietin 1 U group. The number of red blood cells was significantly increased in groups receiving erythropoietin 10 U and 100 U.

Conclusions: Gelatin hydrogel sheets incorporating 1 U erythropoietin improved LV function without inducing polycythemia in a rat chronic myocardial infarction model.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Delayed-Action Preparations
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Echocardiography
  • Erythropoietin / administration & dosage
  • Erythropoietin / pharmacology
  • Erythropoietin / therapeutic use*
  • Gelatin
  • Heart / drug effects
  • Heart / physiology*
  • Male
  • Myocardial Infarction / drug therapy*
  • Myocardial Infarction / physiopathology
  • Neovascularization, Physiologic / drug effects
  • Polycythemia / physiopathology
  • Polycythemia / prevention & control*
  • Rats
  • Rats, Sprague-Dawley
  • Ventricular Dysfunction, Left / drug therapy
  • Ventricular Dysfunction, Left / physiopathology
  • Ventricular Remodeling / drug effects


  • Delayed-Action Preparations
  • Erythropoietin
  • Gelatin