A quantitative kinetic model for ATP-induced intracellular Ca2+ oscillations

J Theor Biol. 2007 Apr 7;245(3):510-9. doi: 10.1016/j.jtbi.2006.11.007. Epub 2006 Nov 16.

Abstract

A quantitative kinetic model is proposed to simulate the ATP-induced intracellular Ca(2+) oscillations. The quantitative effect of ATP concentration upon the oscillations was successfully simulated. Our simulation results support previous experimental explanations that the Ca(2+) oscillations are mainly due to interaction of Ca(2+) release from the endoplasmic reticulum (ER) and the ATP-dependent Ca(2+) pump back into the ER, and the oscillations are prolonged by extracellular Ca(2+) entry that maintains the constant Ca(2+) supplies to its intracellular stores. The model is also able to simulate the sudden disappearance phenomenon of the Ca(2+) oscillations observed in some cell types by taking into account of the biphasic characteristic of the Ca(2+) release from the endoplasmic reticulum (ER). Moreover, the model simulation results for the Ca(2+) oscillations characteristics such as duration, peak [Ca(2+)](cyt), and average interval, etc., lead to prediction of some possible factors responsible for the variations of Ca(2+) oscillations in different types of cells.

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Calcium / metabolism*
  • Computer Simulation*
  • Endoplasmic Reticulum / metabolism
  • Intracellular Fluid / metabolism*
  • Models, Biological
  • Models, Chemical*
  • Protein Kinase C / metabolism
  • Second Messenger Systems / physiology*
  • Type C Phospholipases / metabolism

Substances

  • Adenosine Triphosphate
  • Protein Kinase C
  • Type C Phospholipases
  • Calcium